The production of ligninolytic enzymes by the white-rot fun-us Phanerochaete chrysosporium BKM-F-1767 was studied in a new bioreactor configuration based on a standard rotating drum bioreactor. P. chrysosporium was grown on cubes of nylon sponge, and cultivation was carried out in batch. Two aeration levels: 0.5 and 1 vvm were tested. The latter led to activities about 3-fold higher than the former, achieving maximum manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) activities of 1350 and 364 U/l, respectively. Moreover, laccase activity was also detected, showing a highest activity of 56 U/l. In addition, the in vitro decolorisation of a model dye (Poly R-478) by the extracellular liquid obtained in the bioreactor was monitored in order to assess its ligninolytic ability. A percentage of Poly R-478 decolorisation of about 19% was achieved, after 15 min of dye incubation.