Actinobacillus ureae (A. ureae), is a human pathogen readily detected as an environmental isolate. Presently, no vaccine against A. ureae has been developed yet; thus, a host environment cleaning using disinfectants is the most effective way to prevent the pathogen's transmission. The aim of this study is to determine the most effective disinfectant to inhibit A. ureae, directly extracted from the environment using three easily available disinfectants: 3% hydrogen peroxide (3% HP), 70% isopropyl alcohol (70% IA), and synthetic phenol (SP). Results show that A. ureae is resistant to both 70% IA and SP - which are biocidal to ATCC strains (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger) - in the suspension test. On the other hand, this resistance was not observed with 3% HP. Also in the zone inhibition test, A. ureae is found to be susceptible to 3% HP. Thus, 3% HP showed the most promise for disinfecting A. ureae. (C) 2007 Elsevier Ltd. All rights reserved.