The aim of this study is to isolate a novel fungal pigment from Fusarium moniliforme, so F. moniliforme KUMBF1201 a soil isolate from paddy field has been selected in this study. A new 2-(4-((3E,5E)-14-aminotetradeca-3,5-dienyloxy)butyl)-1,2,3,4-tetrahydroisoquinolin-4-ol (ATDBTHIQN) compound was isolated and characterized from the ethyl acetate extract of a fungal strain F. monillforme KUMBF1201. The structure of ATDBTHIQN compound was established by detailed spectroscopic analysis by UV, FTIR, MS and NMR. This compound exhibited potent mosquito larvicidal activity against third and fourth instar larvae of Aedes aegypti (LC50 =237.0 and 276.4 ppm, r(2) = 0.972 and 0.975) and Anopheles stephensi (LC50 =335.6 and 258.1 ppm, r(2) =0.987 and 0.977). The outcome suggests that the use of F. moniliforme KUMBF1201 can be rapid, environment friendly mosquito larvicides, which can form a novel approach to development of effective biolarvicides for controlling the target vector. (C) 2015 Elsevier Ltd. All rights reserved.