This study investigated the inhibitory effect of the 18 kDa glycoprotein isolated from Lactobacillus plantarum L67 on the bisphenol A-mediated inflammatory reaction in RBL-2H3 cells. The RBL-2H3 cells were treated with bisphenol A (50 mu mol) and the glycoprotein isolated from L plantarum L67 (5-100 mu g/ml) for 30 min. Subsequently, the release of histamine and beta-hexosaminidase into the medium and the intra-cellular Ca2+ levels were measured. The activities of PKC and iNOS were measured by western blotting. In addition, the gene expression levels of IL-4 and TNF-alpha were measured by qRT-PCR. In conclusion, the results demonstrated that the degranulation of the BPA-stimulated RBL-2H3 cells and their histamine release were inhibited by the glycoprotein of L. plantarum L67; concurrently, the levels of intracellular Ca2+, the activity of iNOS, and the expression of TNF-alpha and IL-4, two allergy-related cytokines, were also suppressed by the glycoprotein. Taken together, these results indicate that the glycoprotein might help to prevent allergic diseases induced by environmental hormones, such as BPA. (C) 2015 Elsevier Ltd. All rights reserved.