Biomimetic multilevel structured membrane materials have great potential for energy-effi cient chemical separations and biomedical applications. The current study represents a simple, yet effi cient, method to obtain the biomimetic protein separation membrane and controllable cell culture substrate with high stability, selectivity, and antibacterial property. Here, a molecular imprinting methodology is reported to introduce the high-biocompatible protein ovalbumin (Ova) to a multilevel Ag/SiO2/organic based molecularly imprinted membranes (ASO-MIMs), which have made signifi cant achievements in protein identifi cation and controllable growth of liver cells in vitro platform. Interestingly, the relative morphological observations of the adhered cells and in vitro viability tests show no signifi cant difference between the ASO-MIMs binding with 13.6 mg g(-1) Ova (13.6-ASO-MIMs) and bare glass, indicating the excellent biocompatibility of the 13.6-ASO-MIMs. Here, the results on largely enhanced adsorption capacity, perm-selectivity (a values are more than 2.2), regeneration ability (still maintained 90% of the maximum adsorption capacity after 10 cycling operation), and high-performance cell adhesion system (controlled by the binding amount of template protein) are shown, which clearly demonstrates the potential value of this method in smart biomaterials and biosensors.