Er,Cr:YSGG lasers are currently being investigated for disinfecting the root canal treatment. The aim of this study was to compare the effects of various irrigation protocols on push-out bond strength of fiber posts. Fifty maxillary anterior teeth were divided into five groups (n=10) according to the protocol that applied into the post space. Group-1: distilled water, Group-2: 5% NaOCl, Group-3: 2% CHX, Group-4: Er,Cr:YSGG laser (1.5W, 20Hz, 85 air, 75 water, 26.7J/cm(2)), Group-5: Er,Cr:YSGG laser (1.25W, 50Hz, 34 air, 24 water, 12.7J/cm(2)). Fiber posts were cemented with resin cement. The remaining part of the root, three slices were obtained from each specimen and push-out test was performed. One-way ANOVA and Duncan's test at a 5% level of significance were used for the statistical analysis. Post space irradiation with Er,Cr:YSGG laser (1.5W 20Hz, 85 air, 75 water, 26.7J/cm(2)) increases push-out bond strength of fiber post to root canal dentin. Further investigations are needed to establish and optimize ER,Cr:YSGG laser parameters to increase the push-out bond strength of fiber posts.