In this study, beta-cyclodextrin (beta-CD) was used to enhance the extraction of 2,4-dichlorophenol (2,4-DCP) from the aqueous sample in cloud point extraction (CPE) using spectrophotometric method. Several parameters have been investigated with and without the presence of beta-CD modifier, such as pH, equilibration temperature, analyte concentration, and water content in the CPE and CPE-beta CD systems, respectively. Equilibrium data are described by the Langmuir isotherm in both CPE systems with and without beta-CD modifier. The thermodynamic parameters (positive values of Delta H degrees and Delta S degrees, negative values of Delta G degrees) indicate that the solubilization of 2,4-DCP in a polyethylene glycol silicone surfactant (DC193C) is endothermic, entropy gained, and spontaneous in both systems. In the CPE-beta CD system, the results show that the beta-CD modifier is capable of enhancing the extraction of 2,4-DCP pollutant from aqueous samples.