In this paper we report on the electrodeposition-based construction of film of nano-roughened, hierarchical Au microstructures on indium tin oxide (ITO) surface in a controllable manner. It is found that both the nanotexture and the size of such microstructures can be simply controlled by varying the concentration of Au composition or the deposition potential used. Study on time-evolved microstructure growth further reveals that both the size of the microstructures and the surface coverage of the substrate can be tuned in a continuous mode. It is also found that such Au film exhibits high catalytic activity towards the oxidation of H2O2. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.