A photoelectrochemical detector (PECD) was developed for determination of organic compounds in flow injection analysis (FIA) and high performance liquid chromatography (HPLC) based on the extraordinary oxidation power of nanostructured TiO2 photoanodes under UV illumination. The PECD is a simple, small, and compact photoelectrochemical cell consisting of low cost components such as a TiO2 nanostructured photoanode and a UV-LED. Compared with conventional FIA and HPLC selective detectors, such as UV-Vis detector, electrochemical detector and fluorescent detector, the PECD has the advantage of being low cost, non-selective and sensitive. Using the FIA mode, the analytical principle of the PECD was demonstrated by detecting a large variety of organic compounds, such as sugars, amino acids, alcohol, straight chain carboxylic acids, and aromatic carboxylic acid. The PECD was subsequently coupled with a typical FIA and HPLC system for the determination of sugars. For the application in FIA, under the optimum experimental conditions, the linear ranges for glucose and sucrose were 25-600 mu M and 25-500 mu M, respectively, with the same detection limit of 10 mu M. When the PECD was coupled with a HPLC system, the linear range for both glucose and sucrose was 7.5-200 mM with the same detection limit of 1 mM under the optimal experimental conditions. (C) 2010 Elsevier B.V. All rights reserved.