A novel hemoglobin (Hb) electrode has been presented through adsorbing lib onto graphene-Pt nanocomposite. The graphene-Pt with uniform sheet-like structure was obtained by microwave-assisted method. Positions of Soret absorption band showed lib retained its natural configuration after adsorption onto graphene-Pt. The electrochemical behavior of the immobilized Hb was studied by cyclic voltammetry. The immobilized Hb showed a pair of stable and well-defined peaks at the formal potential of -0.26 V. As a model, a kind of novel third generation biosensor for H(2)O(2) was developed. The resultant heme-protein electrode exhibited electrocatalytical responses to the reduction of H(2)O(2), excellent stability, interference and good reproducibility. The presence of graphene enhanced the protein loadings, accelerates interfacial electron transfer and improves thermal stability of the adsorbed protein. (C) 2011 Elsevier B.V. All rights reserved.