The interaction of dacarbazine (DTIC) with salmon sperm double-stranded DNA (ss-ds-DNA) was studied in phosphate buffer (PB) solution of physiological pH 7.4 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at screen-printed carbon electrode (SPCE). UV-vis absorption spectroscopic technique was also employed to probe the interaction between the DTIC and ss-ds-DNA in solution. The binding of DTIC with DNA, immobilized onto the surface of an anodically activated SPCE, was exploited for designing a sensitive biosensor for DTIC. A detection scheme based on a preconcentration and DPV determination at ss-ds-DNA modified SPCE (DNA/SPCE) was proposed for the trace determination of the DTIC in spiked human serum samples. (c) 2014 Elsevier B.V. All rights reserved.