This work demonstrated the potential application of the N, S dual-doped graphene (NS-G) as a novel glucose oxidase (GOD) platform for glucose sensing. The NS-G was prepared by a simple two-step solvothermal method using urea as the N precursor and benzyl disulfide as the S precursor. The morphology and structure of as-prepared NS-G were characterized, and its electrochemical properties were examined. In comparison with the single N-doped graphene (N-G) based sensor, the dual-doped NS-G modified sensor showed significantly improved electrochemical sensing performances for glucose detection due to the synergistic effect of the coupling interactions between N and S heteroatoms that were responsible for the superior electrocatalytic properties. This study suggested that dual-doped graphene with two heteroatoms could be an effective way to greatly improve the electrochemical sensing performances of graphene-based GOD glucose biosensor. (C) 2014 Elsevier B.V. All rights reserved.