A novel electrochemical glucose sensor based on Au@Ag heterogeneous nanorods (Au@Ag heterogeneous NRs) was developed. The Au@Ag heterogeneous NRs exhibited favorable electrocatalytic ability to reduce H2O2 at negative potential of about -0.6 V (vs. SCE). Further, the glucose oxidase (GOx)/Au@Ag heterogeneous NRs modified electrode was constructed as a glucose sensor, which exhibited wide linear range of 0.02-10 mM glucose in O-2-saturated phosphate buffered saline (PBS) at an applied potential of -0.4 V. Additionally, the glucose sensor showed good reproducibility and stability. Further, the proposed sensor showed good selectivity to common interferents (ascorbic acid, uric acid and dopamine), other saccharides, and 0.15 M chloride ions, which could be applicable for real human serum sample analysis. (C) 2015 Elsevier B.V. All rights reserved.