A glassy carbon electrode modified with poly(bromocresol green) was prepared by electropolymerization process for simultaneous determination of uric acid, dopamine and ascorbic acid. The interface morphology and structure of poly(bromocresol green) film were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The simultaneous determination of uric acid (UA), dopamine (DA) and ascorbic acid (AA) in 0.1 M phosphate buffer solution (pH 6.0) was carried out by differential pulse voltammetric technique. Under optimum conditions, the results show that the peaks of three species were well separated. The proposed sensor exhibited linear responses to UA, DA and AA in the ranges of 0.5-200, 0.05-10 and 0.5-1000 mu M, respectively. Moreover, the poly(bromocresol green) modified electrode has been successfully applied to determine UA, DA and AA in human serum samples and vitamin C tablets. (C) 2015 Elsevier B.V. All rights reserved.