A sensitive electrochemical method for determination of daphnetin at reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) was developed. The graphene nanosheets were simply directly deposited onto a GCE surface through cyclic voltammetric reduction of graphene oxide colloidal solution. The redox character of daphnetin at ERGO/GCE was studied systematically and some dynamic parameters were calculated for the first time. The electroanalytical method for determination of daphnetin was established by differential pulse voltammograms (DPV). Under optimum conditions, the response peak currents were linear related with daphnetin concentrations in the range of 5.0 x 10(-8) to 2.0 x 10(-6) mol L-1 with a detection limit of 3.0 x 10(-8) mol L-1. Therefore, the high sensitivity for daphnetin sensing at the proposed electrode was achieved, which was attributed to the introduced graphene films modified on GCE. Additionally, the proposed method could also be used to detect daphnetin in the medicinal capsules and Chinese medicinal herb Zushima with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.