Penicillin G was continuously extracted by an emulsion liquid membrane (ELM) using Amberlite LA-2 as a carrier and ECA 4360J as a surfactant in a countercurrent extraction column of Oldshue-Rushton type. The effects of various operating conditions on the extraction were experimentally investigated and discussed on the basis of our previous work on batch extraction of penicillin G. In addition, we calculated the relative interfacial area using holdup of emulsion phase in the column which was measured throughout the experiments, and it was known that the magnitude of relative interfacial area had a main influence on degree of extraction. About 90% of penicillin G was extracted under specific conditions, which verified the possibility of applying the extraction of penicillin G using an ELM to a continuous process.