Condensation products of 5-substituted phenyl-2-furoyl hydrazide with different monosaccharides D-glucose, D-galactose, D-mannose, D-fucose and D-arabinose were prepared. The anomerization and cyclic-acyclic isomers were investigated by H-1 NMR spectroscopy. The results showed that, except for the D-glucose derivatives, which were in the presence of beta-anomeric forms, all derivatives were in an acyclic Schiff base form. Their antifungal and antitumor activities were studied. The bioassay results indicated that some title compounds showed superior effects over the commercial positive controls.