The objective of the present study is to investigate the influence of CO2 totally or partly replacing steam in a fluidised bed in conditions close to those of a gasifier of a dual fluidised bed. The influence of CO2 substituting inert gas in the biomass feeder is also investigated. All experiments are performed at 850 degrees C and 1.5 bar(a) in a bubbling fluidized bed fed with 3 kg/h woody biomass. For constant biomass feeding rate and H2O + CO2 volume flowrate, the substitution of H2O by CO2 - from 0% to 100% - leads to modification of the gas composition mainly in relation with the water-gas shift reaction. The hydrocarbon and tar yields are not significantly influenced by this substitution. The net conversion of carbon into gas and the cold gas efficiency are neither influenced by the replacement of H2O by CO2. The H-2/CO volume ratio decreases from 1.8 with 100% H2O to 0.5 with 100% CO2. The substitution of inert gas by CO2 in the feeding line induces an increase of total oxidant input in the gasifier. As (CO2 + H2O) input increases, carbon conversion into gas as well as cold gas efficiency both increase. These results show that substitution of H2O by CO2 does not have any interest in a standard biomass to fuel process, except from an adjustment of H-2/CO ratio for a synthesis in which a low ratio is required, such as the direct DME synthesis. However, with an H-2/CO ratio adjustment by addition of external H-2, the substitution of H2O by CO2 has a positive influence on the biofuel yield and on the CO2 net emission for the global process. (C) 2016 Elsevier Ltd. All rights reserved.