Metal oxide semiconductor field-effect transistor (MOSFET)-based direct monitoring of p53 in spiked serum

Yunkwang Oh; Tran Thi Dung; Younghee Lee; Chang-Soo Lee; Moonil Kim

Journal of Industrial and Engineering Chemistry, Vol.37, 95-100, May, 2016

DOI10.1016/j.jiec.2016.03.015 Export Citation

Metal oxide semiconductor field-effect transistor (MOSFET)-based direct monitoring of p53 in spiked serum

Yunkwang Oh^{1, 2}, Tran Thi Dung^{1, 3}, Younghee Lee², Chang-Soo Lee^{1, 3, †}, and Moonil Kim^{1, 3, †}

¹BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon 305-333, Republic of Korea
²Department of Biochemistry, Chungbuk National University, 27 Naegokno, Heungdeok-Gu, Cheongju 361-763, Republic of Korea
³Department of Nanobiotechnology, University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon 305-350, Republic of Korea

E-mail:,

We describe the direct monitoring of p53 in a human serum sample by a metal oxide semiconductor field-effect transistor (MOSFET) biosensor. The biomolecular interaction between DNA and p53 protein was measured using a DNA-modified MOSFET under the condition of physiological ionic strength. Treated with p53 in serum, a MOSFET exhibited a comparable change in the drain current, while no alteration in response to serum spiked with DNA binding-defective p53 was observed, leading to a good agreement between theory and experiment. Our data suggest that a MOSFET device can be potentially used for the determination of p53 in serum samples on the basis of DNA-protein interaction.

Keywords:MOSFET;Biosensor;p53;Tumor suppressor;Serum

[References]

Lane DP, Nature, 358, 15 (1992)
Chen PL, Chen YM, Bookstein R, Lee WH, Science, 250, 1576 (1990)
Chung J, Irwin MS, Curr. Drug Targets, 11, 667 (2010)
Levine A, Cell, 88, 323 (1997)
Pavletich NP, Chambers KA, Pabo CO, Genes Dev., 7, 2556 (1993)
Hussain SP, Harris CC, Mutat. Res., 428, 23 (1999)
Walker DR, Bond JP, Tarone RE, Harris CC, Makalowski W, Boguski MS, Greenblatt MS, Oncogene, 18, 211 (1999)
Strano S, Dell’Orso S, Agostino SD, Fontemaggi G, Sacchi A, Blandino G, Oncogene, 26, 2212 (2007)
Suh YA, Post SM, Elizondo-Fraire AC, Maccio DR, Jackson JG, El-Naggar AK, Pelt CV, Terzian T, Lozano G, Cancer Res., 71, 7168 (2011)
Balogh GA, et al., Int. J. Oncol., 28, 995 (2006)
Shim KS, Kim KH, Park BW, Lee SY, Choi JH, Han WS, Park EB, J. Korean Med. Sci., 13, 44 (1998)
Suwa H, Ohshio G, Okada N, Wang Z, Fukumoto M, Imamura T, Imamura M, Gut, 40, 647 (1997)
Mattioni M, Chinzari P, Soddu S, Strigari L, Cilenti V, Mastropasqua E, BMC Cancer, 13, 62 (2013)
Park J, Nguyen HH, Abdela W, Kim M, Appl. Sci. Converg. Technol., 23, 61 (2014)
Kim JM, Jha SK, Lee DH, Chand R, Jeun JH, Kim YS, J. Ind. Eng. Chem., 18(5), 1642 (2012)
Chand R, Jeun JH, Park MH, Kim JM, Shin IS, Kim YS, J. Ind. Eng. Chem., 21, 126 (2015)
Zayats M, Kharitonov A, Katz E, Buckmann A, Willner I, Biosens. Bioelectron., 15, 671 (2000)
Park H, Kim S, Park K, Lyu H, Lee C, Chung S, Yun W, Kim M, Chung B, Febs Lett., 583, 157 (2009)
Travers AA, Philos. Trans. R. Soc. Lond. Ser. A-Math. Phys. Eng. Sci., 362, 1423 (2004)
Bell S, Klein C, Muller L, Hansen S, Buchner J, J. Mol. Biol., 322, 917 (2002)
Gomes NP, Espinosa JM, Genes Dev., 24, 111 (2010)
Chen Y, Dey R, Chen L, Structure, 18, 246 (2010)
Wei G, Liu G, Liu X, Febs Lett., 543, 16 (2003)
Oh Y, Lee Y, Heath J, Kim M, IEEE Sens. J., 15, 637 (2015)
Nguyen HH, Park J, Kang S, Kim M, Sensors, 15, 10481 (2015)
Han SH, Kim SK, Park K, Yi SY, Park HJ, Lyu HK, Kim M, Chung BH, Anal. Chim. Acta, 665, 79 (2010)
Ishikawa FN, Curreli M, Chang HK, Chen PC, Zhang R, Cote RJ, Thompson ME, Zhou C, ACS Nano, 3, 3969 (2009)
Lee JO, So HM, Jeon EK, Chang H, Won K, Kim YH, Anal. Bioanal. Chem., 390, 1023 (2008)
Cheng S, Hotani K, Hideshima S, Kuroiwa S, Nakanishi T, Hashimoto M, Mori Y, Osaka T, Materials, 7, 2490 (2014)

[1] Lane DP, Nature, 358, 15 (1992)

[2] Chen PL, Chen YM, Bookstein R, Lee WH, Science, 250, 1576 (1990)

[3] Chung J, Irwin MS, Curr. Drug Targets, 11, 667 (2010)

[4] Levine A, Cell, 88, 323 (1997)

[5] Pavletich NP, Chambers KA, Pabo CO, Genes Dev., 7, 2556 (1993)

[6] Hussain SP, Harris CC, Mutat. Res., 428, 23 (1999)

[7] Walker DR, Bond JP, Tarone RE, Harris CC, Makalowski W, Boguski MS, Greenblatt MS, Oncogene, 18, 211 (1999)

[8] Strano S, Dell’Orso S, Agostino SD, Fontemaggi G, Sacchi A, Blandino G, Oncogene, 26, 2212 (2007)

[9] Suh YA, Post SM, Elizondo-Fraire AC, Maccio DR, Jackson JG, El-Naggar AK, Pelt CV, Terzian T, Lozano G, Cancer Res., 71, 7168 (2011)

[10] Balogh GA, et al., Int. J. Oncol., 28, 995 (2006)

[11] Shim KS, Kim KH, Park BW, Lee SY, Choi JH, Han WS, Park EB, J. Korean Med. Sci., 13, 44 (1998)

[12] Suwa H, Ohshio G, Okada N, Wang Z, Fukumoto M, Imamura T, Imamura M, Gut, 40, 647 (1997)

[13] Mattioni M, Chinzari P, Soddu S, Strigari L, Cilenti V, Mastropasqua E, BMC Cancer, 13, 62 (2013)

[14] Park J, Nguyen HH, Abdela W, Kim M, Appl. Sci. Converg. Technol., 23, 61 (2014)

[15] Kim JM, Jha SK, Lee DH, Chand R, Jeun JH, Kim YS, J. Ind. Eng. Chem., 18(5), 1642 (2012)

[16] Chand R, Jeun JH, Park MH, Kim JM, Shin IS, Kim YS, J. Ind. Eng. Chem., 21, 126 (2015)

[17] Zayats M, Kharitonov A, Katz E, Buckmann A, Willner I, Biosens. Bioelectron., 15, 671 (2000)

[18] Park H, Kim S, Park K, Lyu H, Lee C, Chung S, Yun W, Kim M, Chung B, Febs Lett., 583, 157 (2009)

[19] Travers AA, Philos. Trans. R. Soc. Lond. Ser. A-Math. Phys. Eng. Sci., 362, 1423 (2004)

[20] Bell S, Klein C, Muller L, Hansen S, Buchner J, J. Mol. Biol., 322, 917 (2002)

[21] Gomes NP, Espinosa JM, Genes Dev., 24, 111 (2010)

[22] Chen Y, Dey R, Chen L, Structure, 18, 246 (2010)

[23] Wei G, Liu G, Liu X, Febs Lett., 543, 16 (2003)

[24] Oh Y, Lee Y, Heath J, Kim M, IEEE Sens. J., 15, 637 (2015)

[25] Nguyen HH, Park J, Kang S, Kim M, Sensors, 15, 10481 (2015)

[26] Han SH, Kim SK, Park K, Yi SY, Park HJ, Lyu HK, Kim M, Chung BH, Anal. Chim. Acta, 665, 79 (2010)

[27] Ishikawa FN, Curreli M, Chang HK, Chen PC, Zhang R, Cote RJ, Thompson ME, Zhou C, ACS Nano, 3, 3969 (2009)

[28] Lee JO, So HM, Jeon EK, Chang H, Won K, Kim YH, Anal. Bioanal. Chem., 390, 1023 (2008)

[29] Cheng S, Hotani K, Hideshima S, Kuroiwa S, Nakanishi T, Hashimoto M, Mori Y, Osaka T, Materials, 7, 2490 (2014)