The selective vapour-phase oxidation of 2-MN to 2-MNQ was investigated effectively in the presence of a composite vanadium catalyst. The catalyst was prepared by impregnation. It was characterized by XRD, BET, SEM, etc. The activity of the catalyst was tested in a fixed bed reactor by the selective oxidation of 2-MN to 2-MNQ, and the process conditions were optimized. The proper reaction temperature was 663K, the space velocity was 200mL(gmin)(-1), and the material flow was 3Lmin(-1). The 2-MN conversion rate was 80.01 %, and the yield of 2-MNQ was 45.84 %. Compared to previous procedures for this type of reaction, the reaction is more environmentally friendly and is suitable for large-scale industrial production.