The method for preparation of Co-Mo catalysts for one-stage hydrotreating of full FCC gasoline fraction is proposed. The main characteristic of the catalysts is the use of the supports based on amorphous aluminosilicate. Catalysts were characterized with nitrogen adsorption-desorption, HRTEM and XPS methods. It was shown that all catalysts containing aluminosilicates have similar textural characteristics and contain Co-Mo-S phase with similar morphology. The IR CO data indicate that sulfided catalysts contain different acid sites with the predominance of weak Lewis acid sites and Bronsted acid sites with the strength of 1190 kJ/mol. Catalysts were tested in hydrotreating of model fuel and full FCC gasoline fraction. It was shown that presence of amorphous aluminosilicates in catalysts results in the increase of catalytic activity in reactions of olefin isomerisation. Significant contribution of olefin isomerization reactions results in the prevention of octane number loss while high activity hydrodesulfurisation maintains. (C) 2016 Elsevier B.V. All rights reserved.