In the following study attempts were made to present a novel and sensitive strategy for sensing and determining sertraline. To reach the goal of the study therefore, nano-structured Ni(11)-LD (LD: levodopa) film was electropolymerized on glassy carbon electrode (GCE) which was modified by gold nanoparticles (Au NPs) enriched multi-walled carbon nanotubes (MWCNTs) in alkaline solution. Double-pulse electrochemical technique was applied for electrodeposition of Au NPs on MWCNTs which were immobilized on glassy carbon electrode surface. In the next step, the prepared Au NPs/MWCNT/GCE was modified with Ni (11)-LD film by using cyclic voltammetry technique. Structure of Ni (II)-LD/Au NPs/MWCNT/GCE was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). Furthermore, the electrochemical behavior of Ni (II)-LD/Au NPs/MWCNTs composite and oxidation of sertraline in alkaline solutions was investigated by cyclic voltammetry (CV). It was found that the prepared Ni(II)-LD/Au NPs/MWCNTs nanocomposite, due to its unique properties, reveals high electrocatalytic activity towards oxidation of sertraline. Differential pulse voltammetry (DPV) was used for determining sertraline in the range of 0.05-5.5 ',JIM with a good sensitivity (16.128 mu A/mu M) and a low detection limit of 95 nM (for S/N =3). Finally, the developed sensor indicated several advantages, such as high stability, short time response and excellent reproducibility. (C) 2016 Elsevier Ltd. All rights reserved.