In the current study, a modified single chambered multi-electrode microbial fuel cell was constructed using carbon electrodes, which produced a maximum power density of 25 mW/m(2). Four electrochemically active bacteria were obtained from the biofilm of the single chambered microbial fuel cell operated with Capra hircus rumen fluid collected from a slaughter house. These bacteria were characterized using 16S rRNA analysis and have been identified as Cloacibacterium normanense strain RA1, Micrococcus luteus strain RA2, Diaphorobacter oryzae strain RA3, and Pseudomonas aeruginosa strain RA5. Cloacibacterium normanense strain RA1 showed a steady increase in power density till the 6th day and reached a maximum of 51 mW/m(2) on the same day. The electron transferability of anodic biofilm was studied using cyclic voltammetry. It was found that C. normanense strain RA1, D. oryzae strain RA3, and P. aeruginosa strain RA5 showed oxidation and reduction potential while M. luteus strain RA2 showed only reduction potential.