Effects of thermal treatments on breakage and calcium release of fish bone particles during high-energy wet ball milling were investigated. Heating temperature (55-130 degrees C) showed much more obvious influences on the breakage and calcium release than heating time (20-60 min). As heating temperature increased from 55 degrees C to 120 degrees C, fish bone matrix became more porous, and mechanical parameters of fish bone remarkably decreased (p < 0.05). Furthermore, particle size of the fish bone after milling reached a limit (approximate 110 nm) in a shorter time and calcium release was enhanced. Regardless of heating time, size of the fish bone particles was not significantly different (p > 0.05) between the samples treated with 120 degrees C and 130 degrees C during milling, while calcium release was higher for the latter sample. Breakage kinetics and calcium release of fish bone particles were fitted with the first-order exponential decay function and the Higuchi equation, respectively. (C) 2016 Elsevier Ltd. All rights reserved.