A vertically aligned carbon nanofiber-based (VACNF) electrode platform was developed for an enzyme less hydrogen peroxide sensor. Vertical nanofibers have heights on the order of 2-3 mu m, and diameters that vary from 50 to 100 nm as seen by atomic force microscopy. The VACNF was grown as individual, vertically, and freestanding structures using plasma-enhanced chemical vapor deposition. The electrochemical sensor, for the hydrogen peroxide measurement in solution, showed stability and reproducibility in five consecutive calibration curves with different hydrogen peroxide concentrations over a period of 3 days. The detection limit was 66 mu M. The sensitivity for hydrogen peroxide electrochemical detection was 0.0906 mA cm(-2) mM(-1), respectively. The sensor was also used for the measurement of hydrogen peroxide as the by-product of the reaction of cholesterol with cholesterol oxidase as a biosensor application. The sensor exhibits linear behavior in the range of 50 mu M-1 mM in cholesterol concentrations. The surface analysis and electrochemistry characterization is presented. (C) 2016 Published by Elsevier B.V.