In this paper, the preparation of graphene from graphite oxide (GO) by high gravity technology in Rotating Packed Bed (RPB) was studied. It was verified that the surface area of graphene oxide exfoliated by high gravity technology was about 7-8 times of that obtained by ultrasonic technology and the layer numbers could be reduced to about two layers with high gravity technology. After reduction, the electric conductivity and specific capacitance of graphene prepared by high gravity technology increased 12.5% and 15.0% compared to that prepared by common method respectively. This technology has obvious advantages in sufficient exfoliation and reduction besides better product qualities and application properties. Moreover, it is easily scaled up. (C) 2016 Elsevier B.V. All rights reserved.