In the present investigation, some key fuel properties such as acid value (AV), iodine value (I), peroxide value (PV), water content (WC), and flash point (FP) of cottonseed oil methyl esters (CSOME) and palm stearin methyl esters (PSME) biodiesels, and their blends with petroleum diesel are estimated as per American Society for Testing Materials (ASTM) and American Oil Chemists Society (AOCS) methods. The variations of all the properties with blends have been analyzed as a function of fatty acid profile of biodiesels. The fatty acid profile of the biodiesels is determined using gas chromatography-mass spectrometer (GC-MS) method. CSOME biodiesel is rich in unsaturated fatty acid methyl esters (FAME) and PSME in saturated FAME. Both the biodiesel blends have shown different types of variations for different properties.