In this study, nitrogen and sulfur co-doped mesoporous carbon (N-S-MPC) materials are selected as the platform to demonstrate the potential of N-S-MPC to replace precious metal catalyst for fuel cell cathode oxygen reduction. Using both N-S-MPC and commercial available 40%Pt/C as cathode catalysts, the effects of catalyst and bonding layer in the catalyst layer (CL) on the power generation performances are thoroughly investigated for alkaline membrane fuel cells (AMFCs). Through single cell tests, several observations are reached as follows: (1) For N-S-MPC cathode, with increasing N-S-MPC loading from 1.00 to 5.00 mg cm(-2), the power density reached the maximum (21.7 mW cm(-2)) when the catalyst loading is 3 mg cm(-2). However, for Pt/C cathode the power density reached the maximum (21.3 mW cm 2) for a catalyst loading of 0.5 mg cm(-2), with increasing loading from 0.3 to 0.5 mg cm(-2); (2) Increasing the thickness of catalyst layer resulted in an increase in power density. Thus, raising the local hydroxyl ion concentration was in favor of the process of oxygen reduction reaction. (3) The bonding layer also has a significant influence on the MEA fabrications, where the MEA using 30 mu L bonding layer produced a maximum power density of 20.8 mW cm(-2). (C) 2015 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.