We report on the time evolution of the optical activity of a thinning liquid film containing glucose, and confined between two glass slides. This dynamics strongly depends on the presence of surfactant molecules. With sodium dodecyl sulfate (SDS), we evidence favorable interactions of sugar molecules with the sulfate group. As previously observed for a freely suspended soap film in the air (see Emile et al., 2013), this corresponds to an anchoring of glucose molecules at the interface. For glucose alone, we also highlight a molecular rearrangement that is not instantaneous and occurs after several minutes. This interfacial organization leads to an unusual giant optical activity that is different with or without SDS. Molecular simulations confirm the anchoring of the glucose molecules at the glass liquid interface, and show a different molecular orientation in each case. (C) 2016 Elsevier Inc. All rights reserved.