The emergence of antimicrobial resistant bacteria is an important public health and environmental contamination issue. Antimicrobials of P-lactam group accounts for approximately two thirds, by weight, of all antimicrobials administered to humans due to high clinical efficacy and low toxicity. This study explores beta-lactam resistance determinant gene (bla(TEM)) as emerging contaminant in Indo-Gangetic region using qPCR in molecular beacon format. Quantitative Microbial Risk Assessment (QMRA) approach was adopted to predict risk to human health associated with consumption/exposure of surface water, potable water and street foods contaminated with bacteria having bla(TEM) gene. It was observed that surface water and sediments of the river Ganga and Gomti showed high numbers of bla(TEM) gene copies and varied significantly (p < 0.05) among the sampling locations. The potable water collected from drinking water facility and clinical settings exhibit significant number of bla(TEM) gene copies (13 +/- 0.44-10200 +/- 316 gene copies/100 mL). It was observed that E.crassipes among aquatic flora encountered in both the rivers had high load of bla(TEM) gene copies. The information on prevalence of environmental reservoirs of bla(TEM) gene containing bacteria in Indo-Gangetic region and risk associated will be useful for formulating strategies to protect public from menace of clinical risks linked with antimicrobial resistant bacteria. (C) 2016 Elsevier B.V. All rights reserved.