Introducing the permanganate chemical etching procedure, a new spherulite investigation method of scanning electron microscope(SEM) is developed for the observation of isotactic polypropylene(iPP). New chemical etching conditions for iPP spherulite are as follows; etchant formulation is 3 wt% KMnO₄(s)/64.7 wt% H₂SO₄/32.3 wt% H₃PO₄ and etching time is 7 hours at room temperature. The spherulite morphology of iPP can be observed clearly by an SEM α-Spherulite and β-spherulite can be characterized and classified by the morphology contrast and lamellar growth pattern which are revealed after permanganate etching.