화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.33, No.9, 2575-2581, September, 2016
DOI10.1007/s11814-016-0101-y  Export Citation
Optimization of algal lipid extraction by mixture of ethyl acetate and ethanol via response surface methodology for biodiesel production
Weidong Lu1, 2, Md Asraful Alam2, Ying Pan2, 3, William Junior Nock4, Zhongming Wang2, 5, †, and Zhenhong Yuan2, 5
  • 1School of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China
  • 2Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China
  • 3Nano Science and Technology Institute, University of Science and Technology China, Suzhou 215123, China
  • 4Faculty of Engineering and the Environment, University of Southampton, Southampton, SO17 1BJ, UK
  • 5, China
E-mail:
The effects of extraction time, extraction temperature, solvent to biomass ratio and solvent composition on lipid yield from lyophilized Chlorococcum sp. biomass using a mixture of ethyl acetate and ethanol (EAE), a new proposed solvent, were studied. Subsequently, the process conditions of extraction by EAE were optimized using Box-Behnken design (BBD). The results revealed that the extraction temperature had the greatest effect on lipid extraction efficiency, followed by volume ratio of ethyl acetate to ethanol (EA/E) and extraction time. The largest lipid extraction yield of 15.74% was obtained under the following extraction conditions: 40mL solvents per gram of biomass for 270 min with gentle stirring at 80 ℃ by EAE with an EA/E of 1.0. Furthermore, palmitic acid, stearic acid, oleic acid, and linoleic acid were the most abundant fatty acids in the lipids extracted, indicating the great potential of the proposed lipid extraction procedure for microalgae-based biodiesel production.
Keywords:Biomass;Lipids;Microalgae;Biodiesel
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