In this research, Daturametel Linn seed oil was investigated for the first time as a promising non conventional feedstock for preparation of biodiesel fuel. The maximum extraction of oil was observed to be 38.57 (wt) % and the activation energy Ea (25.8 kJ mol(-1)) was calculated. Further the thermodynamic properties for oil extraction were determined as activation enthalpy = 25.051 kJ mol(-1), activation entropy = -241.25 J mol(-1), and the Gibb's energy = 105.39 kJ mol(-1). This present study also reports the new single-step ultrasound production of biodiesel from high fatty acid D.metel Linn oil using sulfuryl chloride as catalysts in which 95.50% yield was achieved with 2 h. Different reaction parameters for oil extraction and biodiesel production were optimized. This analysis confirms that Daturametel Linn biodiesel is appropriate alternative to petroleum diesel with recommended fuel properties as per specified standards. (C) 2016 Elsevier Ltd. All rights reserved.