Real-time atomic force microscopy imaging of collagen ultraviolet irradiation

Young-Sang Youn; Sehun Kim

Journal of Industrial and Engineering Chemistry, Vol.42, 15-18, October, 2016

DOI10.1016/j.jiec.2016.07.023 Export Citation

Real-time atomic force microscopy imaging of collagen ultraviolet irradiation

Young-Sang Youn^{1, 2, †}, and Sehun Kim²

¹Nuclear Chemistry Research Division, Korea Atomic Energy Research Institute, 111, Deadeok-daero 989beon-gil, Yuseong-gu, Daejeon 34057, Republic of Korea
²Molecular-Level Interface Research Center, Department of Chemistry, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea

E-mail:

Morphological changes that occur on collagen fibril under ultraviolet (UV) irradiation were directly observed using real-time atomic force microscopy (AFM). By analyzing real-time AFM images as a function of UV irradiation time, we found that the gap region of a collagen fibril was more vulnerable to UV light exposure than was the overlap region. In addition, the degraded collagen fragments in the gap region relocated and began accumulating in the overlap region. Through real-time observation of morphological changes using AFM, we verified that collagen fibrils are directly degraded by UV irradiation. Our findings help reveal the mechanisms of skin aging.

Keywords:Collagen;Atomic force microscopy;Ultraviolet;Aging

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[2] Egbert M, Ruetze M, Sattler M, Wenck H, Gallinat S, Lucius R, Weise JM, J. Dermatol. Sci., 73, 40 (2014)

[3] Wang L, Liang Q, Chen T, Wang Z, Xu J, Ma H, Food Hydrocolloids, 38, 104 (2014)

[4] Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ, N. Engl. J. Med., 337, 1419 (1997)

[5] Sionkowska A, Int. J. Biol. Macromol., 35, 145 (2005)

[6] Hulmes DJ, Essays Biochem., 27, 49 (1992)

[7] Orgel JP, Miller A, Irving TC, Fischetti RF, Hammersley AP, Wess TJ, Structure, 9, 1061 (2001)

[8] Hulmes DJS, J. Struct. Biol., 137(1-2), 2 (2002)

[9] Hulmes DJ, Miller A, Parry DA, Piez KA, Woodhead-Galloway J, J. Mol. Biol., 79, 137 (1973)

[10] Itoh T, Kobayashi M, Hashimoto M, Jpn. J. Appl. Phys., 37, L190 (1998)

[11] Kadler KE, Holmes EF, Trotter JA, Chapman JA, Biochem. J., 316, 1 (1996)

[12] Shoulders MD, Raines RT, Annu. Rev. Biochem., 78, 929 (2009)

[13] Kontomaris SV, Yova D, Stylianou A, Balogiannis G, Scanning, 37, 101 (2015)

[14] Grant CA, Phillips MA, Thomson NH, J. Mech. Behav. Biomed. Mater., 5, 165 (2012)

[15] Stylianou A, Yova D, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 2947 (2013)

[16] Armstrong BK, Kricker A, J. Photochem. Photobiol. B-Biol., 63, 8 (2001)

[17] Fujimori E, Eur. J. Biochem., 152, 299 (1985)

[18] Kaminska A, Sionkowska A, Polym. Degrad. Stabil., 51, 15 (1996)

[19] Miles CA, Sionkowska A, Hulin SL, Sims TJ, Avery NC, Bailey AJ, J. Biol. Chem., 275, 33014 (2000)

[20] Sionkowska A, Polym. Degrad. Stabil., 68, 147 (2000)

[21] Rabotyagova OS, Cebe P, Kaplan DL, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 28, 1420 (2008)

[22] Skopinska-Wisniewska J, Sionkowska A, Kaminska A, Kaznica A, Jachimiak R, Drewa T, Appl. Surf. Sci., 255(19), 8286 (2009)

[23] Metreveli NO, Jariashvili KK, Namicheishvili LO, Svintradze DV, Chikvaidze EN, Sionkowska A, Skopinska J, Ecotox. Environ. Safe., 73, 448 (2010)

[24] Stylianou A, Yova D, Alexandratou E, Petri A, Progress Biomed. Opt. Imaging. Proc. SPIE, 8594, 85940E (2013)

[25] Stylianou A, Yova D, Alexandratou E, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 45, 455 (2014)

[26] Paige MF, Rainey JK, Goh MC, Biophys. J., 74, 3211 (1998)

[27] Paige MF, Rainey JK, Goh MC, Micron, 32, 341 (2001)

[28] Minary-Jolandan M, Yu MF, Biomacromolecules, 10(9), 2565 (2009)

[29] Orgel JP, Irving TC, Miller A, Wess TJ, Proc. Natl. Acad. Sci. U. S. A., 103, 9001 (2006)

[30] Marionnet C, Tricaud C, Bernerd F, Int. J. Mol. Sci., 16(1), 68 (2015)