Submillimeter sized n-channel organic single crystalline nanosheet based on dicyanodistyrylbenzene derivative, (2E, 2'E)-3,3'-(2,5-dimethoxy-1,4-phenylene)bis(2-(5-(4-(trifluoromethyl)phenyl) thiophen-2-yl)acrylonitrile) (Me-4-TFPTA), is developed. Strong pi-pi interaction, hydrogen bonding interactions derived from cyano group (CN) as well as solvent inclusion along the lateral direction play a key role in forming nanosheet morphology. Me-4-TFPTA nanosheets exhibit excellent field-effect electron mobility of up to 7.81 cm 2 v(-1) s(-1).