The pretreatment operations (drying, cell disruption and oil extraction) of microalgae biomass to the lipids extraction are important steps to the product quality and production cost. In this study, the lipids from Spirulina were obtained by different methods of biomass drying (tray and spouted bed), cell disruption (microwave, autoclaving and milling) and solvent extraction (hot and cold). The average content of lipids extracted by the cold method, using polar solvents, was of 5.8 +/- 0.6 g 100 g(-1). The spouted bed drying with cell disruption by milling achieved the best performance with the hot extraction method. Under these conditions, the TBA value was of 0.57 +/- 0.09 mgMDA kg(-1). FT-IR spectra and thermal analysis indicated that the hot extraction resulted in a more purified lipid extract than the cold extraction. The Brimberg model showed the best fit to the lipid extraction kinetics and the activation energy in the best conditions was of 6.11 +/- 1.41 kJ mol(-1). (C) 2016 Elsevier Ltd. All rights reserved.