Advanced biofuels like dimethyl-carbonate (DMC), isobutanol and n-pentanol can be derived from non-food based biomass feedstock without unsettling food supplies and biodiversity. An experimental and statistical investigation was carried out to analyze the effects of injection-timing and exhaust gas recirculation (EGR) on performance and emissions of a DI diesel engine using advanced biofuel/diesel blends (containing 8 wt% oxygen). Engine characteristics were measured under high-load condition using moderate EGR (up to 30%) and injection-timing modification (up to +/- 2 degrees CA bTDC) for controlling charge dilution and combustion-phasing. Multiple regression models developed using response surface methodology (RSM) for measured responses like nitrogen oxides (NOx), smoke opacity and brake specific fuel consumption (BSFC) were found to be statistically significant by analysis of variance (ANOVA). Interactive effects between injection timing and EGR for all blends were analyzed using response surface plots that were fitted using developed models. Optimization was performed using desirability approach of the RSM to minimize NOx and smoke emissions simultaneously with minimum BSFC. Isobutanol/diesel blend injected at 22 degrees CA bTDC without EGR was predicted to be optimum for the tested engine. Confirmatory tests validated that the models developed using RSM are adequate to describe the effects of the injection timing and EGR on performance and emissions characteristics using all blends as the error in prediction is within 5%. (C) 2016 Elsevier Ltd. All rights reserved.