In this work, a sensitive non-enzymatic electrochemical glucose sensor with excellent sensitivity, very low detection limit and low glucose oxidation potential was prepared by electrodeposed of nickel nanostructure (NiNS) supported on the surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS). The determination of glucose concentration is very important in clinical, biological samples and chemicals as well as in food processing and fermentation. Electrochemical activity of the electrode toward the oxidation of glucose was studied using cyclic voltammetry, chronoamperometry, linear sweep voltammetry and amperometry. Electrochemical measurements indicated that the high sensitivity of 6.640 mu A mu M-1 cm(-2) and a detection limit of 0.690 mu M were obtained for glucose. In addition, the interference from the oxidation of uric acid, dopamine, acetaminophen and ascorbic acid could be effectively avoided. Meanwhile, the non-enzymatic glucose sensors exhibited excellent selectivity, stability and repeatability. Thus, this novel non-enzyme sensor has potential application in glucose detection. (C) 2016 Elsevier B.V. All rights reserved.