A roboust aptamer based electrochemical cytosensing platform for detection of adenocarcinoma type lung cancer based on electrochemical impedance spectrometry (EIS) was developed with high sensitivity, selectivity and devoted to medical applications. Screen printed carbon electrodes (SCPEs) were used as sensor surface, 5'amino linked aptamer sequence was immobilized onto the SPCEs, interaction with cancer cells at 37.5 degrees C was monitored by EIS transduction of the Rct in the presence of 5 mM [Fe(CN)(6)](3-/4-). Designed aptasensor's selectivity was determined by using Human liver hepatocellular carcinoma (HepG2) and human cervical cancer (HeLA) cells. The purposed cytosensor showed high sensitivity with a detection limit of 163.7 cells/mL. This simple, rapid and low cost electrochemical approach offered a label free detection with capable of early detection in cancer diagnosis with interest for future applications. (C) 2016 Published by Elsevier B.V.