Recently, the use of nano-patterned or nano-structured materials have become widespread in various research areas and applications. However, the fabrication of uniform and sub-50-nm structures over a large, non-flat area is still difficult and time-consuming with the current technology. One method to overcome such hurdles is to use amphiphilic block copolymers that can self-assemble to form micro-or nano-structures and is suitable for large-scale industrial applications. In this study, we synthesized amphiphilic block copolymers with different chemical compositions or molecular weights and could successfully make uniform nanostructures with 15-50-nm-wide dot-like domains without annealing. Moreover, it was confirmed that changing the chemical composition reversed the phase of structures, and the size of dot-like domains could be controlled by changing the molecular weights. Such controllable and reversible nanostructures may find many uses in various fields, including adhesion-controlled tissue engineering scaffolds and orientation-controlled antibody-based immunoassays. (C) 2016 Elsevier Ltd. All rights reserved.