Aspergillus niger NRRL 3112 can produce considerable amounts of beta-1,4-glucosidase (BGL) when grown on wheat bran and glycerol as co-substrates. BGL production was first investigated in a stirred-tank bioreactor (STR) at 450 rpm and 2 vvm. About 5.4 U/mL BGL was obtained using spore suspension as inoculum, whereas a higher production of 9.3 U/mL was obtained using precultured cell pellets. The production of BGL in batch, fed-batch and repeated batch modes in a rotating fibrous bed bioreactor (RFBB) was then studied and compared to the STR. The highest BGL productivity of 1.78 U/mL/day was obtained in the RFBB operated at a repeated batch mode, which was slightly higher than that (1.65 U/mL/day) obtained in the STR with preformed cell pellets and about 1.75-fold of that (1.02 U/mL/day) for the free cell fermentation in STR inoculated with spores. This work demonstrated that the RFBB could provide an efficient process for beta-glucosidase production from low-cost wheat bran and glycerol. (C) 2016 Elsevier Ltd. All rights reserved.