화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.43, 13-19, November, 2016
DOI10.1016/j.jiec.2016.07.054  Export Citation
Biosynthesis of a novel fisetin glycoside from engineered Escherichia coli
Ramesh Prasad Pandey1, 2, Prakash Parajuli2, Luan Luong Chu2, Seung-Young Kim1, 2, and Jae Kyung Sohng1, 2, †
  • 1Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Republic of Korea
  • 2Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Republic of Korea
E-mail:
Escherichia coli BL21(DE3)/DpgiDzwfDgalU mutant was engineered by overexpressing thymidine diphosphate (dTDP)-D-glucose synthase (tgs), dTDP-D-glucose 4,6-dehydratase (dh), and a sugar aminotransferase (wecE) from different sources to produce a pool of dTDP-4-amino-4,6-dideoxy-Dgalactose in the cell cytosol. To this recombinant mutant, two Arabidopsis thaliana glycosyltransferases (ArGT-3 and ArGT-4) were overexpressed to generate two glycosylation platforms (E. coli BL21(DE3)/ΔpgiΔzwfΔgalUTDW-3 and E. coli BL21(DE3)/ΔpgiΔzwfΔgalUTDW-4), which were accessed for the glycosylation of fisetin. As a result, one of the two systems, E. coli BL21(DE3)/ΔpgiΔzwfΔgalUTDW-3, was able to conjugate 4-amino-4,6-dideoxy-D-galactose sugar at the 3-OH position of fisetin, producing an unnatural fisetin 3-O-4-amino-4,6-dideoxy-D-galactoside.
Keywords:Fisetin;Fisetin aminoglycoside;Biotransformation;Unnatural;flavonoid glycoside;E. coli
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