Numerical study on the complete blood cell sorting using particle tracing and dielectrophoresis in a microfluidic device

Haider Ali; Cheol Woo Park

Korea-Australia Rheology Journal, Vol.28, No.4, 327-339, November, 2016

DOI10.1007/s13367-016-0033-4 Export Citation

Numerical study on the complete blood cell sorting using particle tracing and dielectrophoresis in a microfluidic device

Haider Ali, and Cheol Woo Park^†

School of Mechanical Engineering, Kyungpook National University, Daegu 41566, Republic of Korea

E-mail:

In this study, a numerical model of a microfluidic device with particle tracing and dielectrophoresis fieldflow fractionation was employed to perform a complete and continuous blood cell sorting. A low voltage was applied to electrodes to separate the red blood cells, white blood cells, and platelets based on their cell size. Blood cell sorting and counting were performed by evaluating the cell trajectories, displacements, residence times, and recovery rates in the device. A novel numerical technique was used to count the number of separated blood cells by estimating the displacement and residence time of the cells in a microfluidic device. For successful blood cell sorting, the value of cells displacement must be approximately equal to or higher than the corresponding maximum streamwise distance. The study also proposed different outlet designs to improve blood cell separation. The basic outlet design resulted in a higher cells recovery rate than the other outlets design. The recovery rate decreased as the number of inlet cells and flow rates increased because of the high particle-particle interactions and collisions with walls. The particle-particle interactions significantly affect blood cell sorting and must therefore be considered in future work.

Keywords:blood transfusion;cell sorting;particle tracing;dielectrophoresis;recovery rate

[References]

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Basabe-Desmonts L, Ramstrom S, Meade G, O'Neill S, Riaz A, Lee LP, Ricco AJ, Kenny D, Langmuir, 26(18), 14700 (2010)
Gourikutty BN, Chang SCP, Puiu PD, J. Chromatogr. B, 1011, 77 (2016)
Braschler T, Demierre N, Nascimento E, Silva T, Oliva AG, Renaud P, Lab Chip, 8, 280 (2008)
Cetin B, Li DQ, Electrophoresis, 32(18), 2410 (2011)
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Demierre N, Braschler T, Muller R, Renaud P, Sens. Actuators B-Chem., 132, 388 (2008)
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Furlan JC, Fang J, Silver FL, J. Neurol. Sci., 362, 198 (2016)
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Hughes MP, Electrophoresis, 23(16), 2569 (2002)
Lapizco-Encinas BH, Simmons BA, Cummings EB, Fintschenko Y, Anal. Chem., 76, 1571 (2004)
Lin HK, Zheng S, Williams AJ, Balic M, Groshen S, Scher HI, Fleisher M, Stadler W, Datar RH, Tai YC, Cote RJ, Clin. Cancer Res., 16, 5011 (2010)
Mathew B, Alazzam A, Abutayeh M, Gawanmeh A, Khashan S, Chem. Eng. Sci., 138, 266 (2015)
Mernier G, Piacentini N, Tornay R, Buffi N, Renaud P, Sens. Actuators B-Chem., 154, 160 (2011)
Park S, Zhang Y, Wang TH, Yang S, Lab Chip, 11, 2893 (2011)
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Pommer MS, Zhang YT, Keerthi N, Chen D, Thomson JA, Meinhart CD, Soh HT, Electrophoresis, 29(6), 1213 (2008)
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Slichter SJ, Harker LA, Br. J. Haematol., 34, 395 (1976)
Stroncek DF, Rebulla P, Lancet, 370, 427 (2007)
Tatsumi K, Kawano K, Okui H, Shintani H, Nakabe K, Med. Eng. Phys., 38, 24 (2016)
Tornay R, Braschler T, Demierre N, Steitz B, Finka A, Hofmann H, Hubbell JA, Renaud P, Lab Chip, 8, 267 (2008)
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[1] Ali H, Cheema TA, Yoon HS, Do Y, Park CW, Biotechnol. Bioeng., 112(2), 297 (2015)

[2] Bakker E, Qattan M, Mutti L, Demonacos C, Krstic-Demonacos M, Biochim. Biophys. Acta-Mol. Cell Res., 1863, 414 (2016)

[3] Basabe-Desmonts L, Ramstrom S, Meade G, O'Neill S, Riaz A, Lee LP, Ricco AJ, Kenny D, Langmuir, 26(18), 14700 (2010)

[4] Gourikutty BN, Chang SCP, Puiu PD, J. Chromatogr. B, 1011, 77 (2016)

[5] Braschler T, Demierre N, Nascimento E, Silva T, Oliva AG, Renaud P, Lab Chip, 8, 280 (2008)

[6] Cetin B, Li DQ, Electrophoresis, 32(18), 2410 (2011)

[7] Chen L, Zheng XL, Hu N, Yang J, Luo HY, Jiang F, Liao YJ, Chin. J. Anal. Chem., 43, 300 (2015)

[8] Cheng JL, Han SF, Li YQ, Chu YP, Sun YM, Guo JF, Chin. Nurs. Res., 2, 89 (2015)

[9] Costantini V, Zacharski LR, Moritz TE, Edwards RL, Thromb. Haemost., 64, 501 (1990)

[10] Dash S, Mohanty S, Pradhan S, Mishra BK, J. Taiwan Inst. Chem. Eng., 58, 39 (2016)

[11] Demierre N, Braschler T, Muller R, Renaud P, Sens. Actuators B-Chem., 132, 388 (2008)

[12] Egger M, Donath E, Biophys. J., 68, 364 (1995)

[13] Furlan JC, Fang J, Silver FL, J. Neurol. Sci., 362, 198 (2016)

[14] Gascoyne P, Satayavivad J, Ruchirawat M, Acta Trop., 89, 357 (2004)

[15] Geddes LA, Baker LE, Med. Biol. Eng. Comput, 5, 271 (1967)

[16] Han KH, Frazier AB, Lab Chip, 8, 1079 (2008)

[17] Hughes MP, Electrophoresis, 23(16), 2569 (2002)

[18] Lapizco-Encinas BH, Simmons BA, Cummings EB, Fintschenko Y, Anal. Chem., 76, 1571 (2004)

[19] Lin HK, Zheng S, Williams AJ, Balic M, Groshen S, Scher HI, Fleisher M, Stadler W, Datar RH, Tai YC, Cote RJ, Clin. Cancer Res., 16, 5011 (2010)

[20] Mathew B, Alazzam A, Abutayeh M, Gawanmeh A, Khashan S, Chem. Eng. Sci., 138, 266 (2015)

[21] Mernier G, Piacentini N, Tornay R, Buffi N, Renaud P, Sens. Actuators B-Chem., 154, 160 (2011)

[22] Park S, Zhang Y, Wang TH, Yang S, Lab Chip, 11, 2893 (2011)

[23] Paul B, Nesbitt ID, Surgery, 31, 59 (2013)

[24] Piacentini N, Mernier G, Tornay R, Renaud P, Biomicrofluidics, 5, 1 (2011)

[25] Pommer MS, Zhang YT, Keerthi N, Chen D, Thomson JA, Meinhart CD, Soh HT, Electrophoresis, 29(6), 1213 (2008)

[26] Radulescu IR, Candea D, Halanay A, Math. Comput. Simul., 121, 1 (2016)

[27] Reschiglian P, Zattoni A, Roda B, Michelini E, Roda A, Trends Biotechnol., 23, 475 (2005)

[28] Sant HJ, Gale BK, 2007, Microscale Field-Flow Fractionation: Theory and Practice, In: Hardt S, Schonfeld F, eds., Microfluidic Technologies for Miniaturized Analysis Systems, Springer US, Boston, 471-521.

[29] Slichter SJ, Harker LA, Br. J. Haematol., 34, 395 (1976)

[30] Stroncek DF, Rebulla P, Lancet, 370, 427 (2007)

[31] Tatsumi K, Kawano K, Okui H, Shintani H, Nakabe K, Med. Eng. Phys., 38, 24 (2016)

[32] Tornay R, Braschler T, Demierre N, Steitz B, Finka A, Hofmann H, Hubbell JA, Renaud P, Lab Chip, 8, 267 (2008)

[33] Voldman J, Annu. Rev. Biomed. Eng., 8, 425 (2006)