사람피부세포에서 카렌둘라 꽃 추출물의 항산화 및 산화적 스트레스에 대한 세포보호효과

현송화; 김가윤; 유지연; 김지원; 양예림; 전영희; 정윤주; 김아랑; 박수남; Song Hua Xuan; Ga Yoon Kim; Ji Yeon Yu; Jee Won Kim; Ye Rim Yang; Young Hee Jeon; Yoon Ju Jeong; A Rang Kim; Soo Nam Park

Applied Chemistry for Engineering, Vol.27, No.6, 620-626, December, 2016

DOI10.14478/ace.2016.1093 Export Citation

사람피부세포에서 카렌둘라 꽃 추출물의 항산화 및 산화적 스트레스에 대한 세포보호효과

Antioxidant and Cellular Protective Effects against Oxidative Stress of Calendula officinalis Flowers Extracts in Human Skin Cells

현송화, 김가윤¹, 유지연¹, 김지원¹, 양예림¹, 전영희¹, 정윤주, 김아랑, 박수남 ^†

서울과학기술대학교 정밀화학과 화장품종합기술연구소, 코스메틱 융⋅복합산업 지원 센터
¹한성과학고등학교

Song Hua Xuan, Ga Yoon Kim¹, Ji Yeon Yu¹, Jee Won Kim¹, Ye Rim Yang¹, Young Hee Jeon¹, Yoon Ju Jeong, A Rang Kim, and Soo Nam Park^†

Department of Fine Chemistry, Cosmetic R&D center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, Korea
¹Hansung Science High School, Seoul 03732, Korea

E-mail:

초록

본 연구에서는 카렌둘라 꽃의 50% 에탄올 추출물과 분획물의 총 페놀과 플라보노이드 함량, 항산화 활성 및 사람피부세포에서 산화적 스트레스에 대한 세포보호효과를 확인하였다. 자유 라디칼 소거 활성(FSC50), 활성산소 소거 활성(총항산화능, OSC50) 및 사람피부세포 내 ROS 억제활성을 통하여 카렌둘라 꽃의 50% 에탄올 추출물 및 분획물들의 항산화 활성을 측정하였다. 그 결과, 카렌둘라 꽃의 50% 에탄올 추출물보다 그것의 에틸아세테이트 및 아글리콘 불획물이 더 큰 항산화 활성을 나타내었다. 세포보호효과 실험에서 과산화수소를 사람피부세포에 처리하여 세포손상을 유도하였을 때, 에틸아세테이트 분획은 0.05-3.13 μg/mL에서 농도 의존적으로 세포보호효과를 나타내었다. 또한, UVB를 사람피부세포에 조사하여 세포손상을 유도하였을 때, 아글리콘 분획은 1.56-3.13 μg/mL에서 농도 의존적으로 세포보호효과를 나타내었다. 이상의 결과들은 산화적 스트레스에 노출된 사람피부세포에서 카렌둘라 꽃의 분획물들이 ROS소거함으로써 세포를 보호하는 천연 항산화제로 화장품에 응용 가능함을 시사하였다.

In this study, we investigated the total phenolic and flavonoid contents, antioxidant activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract and its fractions of Calendula officinalis (C. officinalis) flowers. We measured the antioxidant effects of 50% ethanol extract and its fractions of C. officinalis flowers on the free radical scavenging activity (FSC50), the reactive oxygen species (ROS) scavenging activities (OSC50) and the inhibition of intracellular ROS generation in human skin cells. These results showed that the antioxidant effect of the ethyl acetate and aglycone fraction was more than the 50% ethanol extract of C. officinalis flowers. We also investigated the cellular protective activity and the results showed that treatment of the ethyl acetate fraction (0.05 - 3.13 μg/mL) protects human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with H2O2. In addition, the aglycone fraction (1.56 - 3.13 μg/mL) shows cellular protective effects on the UV-induced cell damages in a dose-dependent manner. These results suggest that the fractions of C. officinalis flowers can function as a natural antioxidant agent of cosmetics in human skin cells exposed to oxidative stress by ROS scavenging effects.

Keywords:Calendula officinalis;antioxidant;reactive oxygen species;cellular protective effect

[References]

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Al-Nuaimi Y, Sherratt MJ, Griffiths CE, Maturitas, 79, 256 (2014)
Wohlrab J, Hilpert K, Wolff L, Hautarzt, 67, 107 (2016)
Park SN, Kim SY, Lim GN, Jo NR, Lee MH, J. Ind. Eng. Chem., 18(2), 680 (2012)
Ha JH, Jeong YJ, Seong JS, Kim KM, Kim AY, Fu MM, Suh JY, Lee NH, Park J, Park SN, J. Soc. Cosmet. Sci. Korea, 41, 361 (2015)
Seong JS, Kim KM, Suh JY, Ha JH, Park SN, J. Korean Oil Chem. Soc., 32, 781 (2015)
Fonseca YM, Catini CD, Vicentini ET, Nomizo A, Gerlach RF, Fonseca MJ, J. Ethnopharmacol., 127, 596 (2010)
Re TA, Mooney D, Antignac E, Dufour E, Bark I, Srinivasan V, Nohynek G, Food Chem. Toxicol., 47, 1246 (2009)
Chandran PK, Kuttan R, J. Clin. Biochem. Nutr., 43, 58 (2008)
Jimenez-Medina E, Garcia-Lora A, Paco L, Algarra I, Collado A, Garrido F, BMC Cancer, 6, 119 (2006)
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Barroso MF, Noronha JP, Delerue-Matos C, Oliveira MB, J. Agric. Food Chem., 59, 5062 (2011)
Park SN, Won DH, Hwang JP, Han SB, J. Ind. Eng. Chem., 20(5), 3046 (2014)
Kim M, Park YG, Lee HJ, Lim SJ, Nho CW, J. Agric. Food Chem., 63, 5428 (2015)
Shin SW, Jung E, Kim S, Kim JH, Kim EG, Lee J, Park D, PLoS One, 8, e61971 (2013)
Silvan JM, Reguero M, de Pascual-Teresa S, Food Funct., 7, 1067 (2016)
Mosmann T, J. Immunol. Methods, 65, 55 (1983)
Rice-Evans CA, Miller NJ, Paganga G, Free Radic. Biol. Med., 20, 933 (1996)
Packer L, New Compr. Biochem., 28, 239 (1994)

[Referenced By]

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[1] Kammeyer A, Luiten RM, Ageing Res. Rev., 21, 16 (2015)

[2] Wlaschek M, Tantcheva-Poor I, Naderi L, Ma WJ, Schneider A, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K, J. Photochem. Photobiol. B-Biol., 63, 41 (2001)

[3] Bernhard D, Moser C, Backovic A, Wick G, Exp. Gerontol., 42, 160 (2007)

[4] Park SN, J. Soc. Cosmet. Sci. Korea, 23, 75 (1997)

[5] Al-Nuaimi Y, Sherratt MJ, Griffiths CE, Maturitas, 79, 256 (2014)

[6] Wohlrab J, Hilpert K, Wolff L, Hautarzt, 67, 107 (2016)

[7] Park SN, Kim SY, Lim GN, Jo NR, Lee MH, J. Ind. Eng. Chem., 18(2), 680 (2012)

[8] Ha JH, Jeong YJ, Seong JS, Kim KM, Kim AY, Fu MM, Suh JY, Lee NH, Park J, Park SN, J. Soc. Cosmet. Sci. Korea, 41, 361 (2015)

[9] Seong JS, Kim KM, Suh JY, Ha JH, Park SN, J. Korean Oil Chem. Soc., 32, 781 (2015)

[10] Fonseca YM, Catini CD, Vicentini ET, Nomizo A, Gerlach RF, Fonseca MJ, J. Ethnopharmacol., 127, 596 (2010)

[11] Re TA, Mooney D, Antignac E, Dufour E, Bark I, Srinivasan V, Nohynek G, Food Chem. Toxicol., 47, 1246 (2009)

[12] Chandran PK, Kuttan R, J. Clin. Biochem. Nutr., 43, 58 (2008)

[13] Jimenez-Medina E, Garcia-Lora A, Paco L, Algarra I, Collado A, Garrido F, BMC Cancer, 6, 119 (2006)

[14] Zitterl-Eglseer K, Sosa S, Jurenitsch J, Schubert-Zsilavecz M, Loggia RD, Tubaro A, Bertoldi M, Franz C, J. Ethnopharmacol., 57, 139 (1997)

[15] Alves RC, Costa ASG, Jerez M, Casal S, Sineiro J, Nunez MJ, Oliveira B, J. Agric. Food Chem., 58, 12221 (2010)

[16] Barroso MF, Noronha JP, Delerue-Matos C, Oliveira MB, J. Agric. Food Chem., 59, 5062 (2011)

[17] Park SN, Won DH, Hwang JP, Han SB, J. Ind. Eng. Chem., 20(5), 3046 (2014)

[18] Kim M, Park YG, Lee HJ, Lim SJ, Nho CW, J. Agric. Food Chem., 63, 5428 (2015)

[19] Shin SW, Jung E, Kim S, Kim JH, Kim EG, Lee J, Park D, PLoS One, 8, e61971 (2013)

[20] Silvan JM, Reguero M, de Pascual-Teresa S, Food Funct., 7, 1067 (2016)

[21] Mosmann T, J. Immunol. Methods, 65, 55 (1983)

[22] Rice-Evans CA, Miller NJ, Paganga G, Free Radic. Biol. Med., 20, 933 (1996)

[23] Packer L, New Compr. Biochem., 28, 239 (1994)