The aim of this work was to study the culture performance of a dinoflagellate in a commercial photobioreactor. The results obtained during this long-term experiment allow to confirm that Amphidinium carterae is a promising dinoflagellate that can be exploited successfully in closed systems, in semi-continuous mode in indoor and outdoor environments. The average results in an indoor 5 cm light-path 320 L photobioreactor were, in terms of specific growth rate (0.29 d(-1)), duplication time (3.1 d(-1)) and dry biomass productivity (78 mg L-1 d(-1)). Specific compounds production was found including omega 3 and omega 6 fatty acids and, pigments (Peridinin, beta-carotene). These promising results, besides unique characteristics found during the exploitation period such as resistance to mechanical stress, self-control of contaminant organisms, and quick cells aggregation when the culture is not in turbulence conditions, makes A. carterae one of the new target species suitable for commercially exploitation on an industrial scale. (C) 2016 Elsevier Ltd. All rights reserved.