Cultivation modes of autotrophic microalgae for biodiesel production utilizing open raceway pond were analyzed in this study. Five before screened good microalgae were tested their lipid productivity and biodiesel quality again in outdoor 1000 L ORP. Then, Chlorella sp. L1 and Monoraphidium dybowskii Y2 were selected due to their stronger environmental adaptability, higher lipid productivity and better biodiesel properties. Further scale up cultivation for two species with batch and semi-continuous culture was conducted. In 40,000 L ORP, higher lipid productivity (5.15 versus 4.06 g m(-2) d(-1) for Chlorella sp. L1, 5.35 versus 3.00 g m(-2) d(-1) for M. dybowskii Y2) was achieved in semi-continuous mode. Moreover, the financial costs of 14.18 $ gal(-1) and 13.31 $ gal(-1) for crude biodiesel in two microalgae with semi-continuous mode were more economically feasible for commercial production on large scale outdoors. (C) 2016 Elsevier Ltd. All rights reserved.