In this study, a Saccharomyces cerevisiae recombinant strain 14 was constructed through genome shuffling method by transferring the whole genomic DNA of Candida intermedia strain 23 into a thermo-tolerant S. cerevisiae strain. The recombinant strain 14 combined the good natures of both parent strains that efficiently produced ethanol from glucose and single cell protein from xylose with 54.6% crude protein and all essential amino acids except cysteine at 35 degrees C. Importantly, the recombinant strain 14 produced 64.07 g/L ethanol from 25% (w/v) NaOH-pretreated and washed corn stover with the ethanol yield of 0.26 g/g total stover by fed-batch simultaneous saccharification and fermentation and produced 66.50 g/L dry cell mass subsequently from the residual hydrolysate and ethanol. Therefore, this study represents a feasible method to comprehensively utilize hexose and pentose in lignocellulosic materials. (C) 2016 Elsevier Ltd. All rights reserved.