Large-scale biomass production and sewage refining for use in landscaping by employing Chlorella vulgaris were investigated. The effects of the concentrations of carbon, nitrogen, and phosphorus as nutrients in the process were optimized by central composite design. In general, the results revealed that higher amounts of biomass can be obtained by varying the feed concentration; both the N and P concentrations have an effective role in improving the biomass dry weight. The amount of lipid productivity varied from 12% to 55%, and one of the photobioreactors tested showed the best conditions for lipid productivity. Also, this process can decrease the greenhouse gas emissions in wastewater treatment.