The integration utilization of fermentation residues from cellulosic bio-ethanol has attracted a great deal of attention to balance the total cost of bio-ethanol production while simultaneously dealing with bio-ethanol wastewater. A process of hydrothermal liquefaction (HTL) of intact materials from cellulosic bio-ethanol in a batch reactor was proposed. The effects of the reaction temperature and time on the liquefaction characteristics were examined. The optimum condition for liquefaction fermentation residues was 370 degrees C (21.25 MPa) and 30 min with a bio-oil yield of 40.79 wt%. GC-MS results indicated that the major chemical species in the bio-oil were phenols, ketones, long-chain hydrocarbons and fatty acids. Supercritical conditions (375 degrees C, 23.50 MPa) was favored for the low-molecular-weight species formation compared to subcritical conditions (370 degrees C, 21.25 MPa), as some long-chain species decreased. This work thus can provide a novel idea for bio-oil production from HTL of cellulosic bio-ethanol fermentation residues. (C) 2016 Published by Elsevier Ltd.