Biodiesel is an eco-friendly fuel source which is produced from various oil sources. In this study, sinensis oil is tried as a biodiesel source. But seed is simply dumped as waste material to the environment. It contains 30-35% of oil content and can be processed by the transesterification method. The fatty acid profile of sinensis oil is analyzed by gas chromatography-mass spectrophotometer techniques. This study also describes the characterization and optimization of sinensis oil to biodiesel. The optimized biodiesel parameters were as follows: KOH catalyst, 6:1 molar ratio, 1.0 wt.% of catalyst concentration, 60 degrees C temperature, and 120 min reaction time.